Genome analysis leads to discovery of Quorum Sensing Genes in Cedecea neteri
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چکیده
We have identified a strain of C. neteri SSMD04 isolated from pickled mackerel sashimi that 11 produced N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity. Tandem mass 12 sspectrometry revealed that C. neteri SSMD04 produced N-butyryl-homoserine lactone (C4-HSL). 13 We identified a pair of luxIR homologues in this genome that shares the highest similarity with croIR 14 from Citrobacter rodentium. The AHL synthase, which we named it as cneI (636 bp) and at 8bp 15 distance from cneI is a sequence encoding a hypothetical protein, potentially the cognate receptor, a 16 luxR homologue which we named it as cneR. We also found an orphan luxR in this isolate. To our 17 knowledge, this is the first report on the AHL production activity in C. neteri, discovery of its luxI/R 18 homologues and the orphan receptor. 19
منابع مشابه
Genome analysis of quorum sensing Cedecea neteri SSMD04 leads to identification of its novel signaling synthase (cneI), cognate receptor (cneR) and an orphan receptor
Cedecea neteri is a very rare human pathogen. We have isolated a strain of C. neteri SSMD04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. Using the biosensor Chromobacterium violaceum CV026, we have demonstrated the presence of short chain N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity in C. neteri SSMD04. Triple quadrupole LC/MS analysi...
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